Dr Jeff Kuret

108A Rightmire Hall
1060 Carmack Road
Columbus, OH 43210

Phone: (614) 688-5899
Fax: (614) 292-5379

Email: kuret.3@osu.edu

Current OSU Appointment

Professor, Molecular & Cellular Biochemistry

Chapters In Books

Kuret, J., Murad, F. 1990. Adenohypophyseal hormones and related substances. In Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th edition.. Edited by Gilman, A.G., Goodman, L.S., Rall, T.W., Taylor, P. New York: Macmillan.

Murad, F., Kuret, J. 1990. Estrogens and Progestins. In Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th edition. Edited by Gilman, A.G., Goodman, L.S., Rall, T.W., Taylor, P. New York: Macmillan.

Kuret, J. 2007. Detection and Reduction of Neurofibrillary Lesions. In Protein Folding Diseases: Enzyme Inhibitors and Other Agents as Prospective Therapies. Enzyme Inhibitors. Vol. Vol. 5. Edited by H. John Smith, Robert D.E. Sewell and Claire Simons. Boca Raton, Florida: CRC Press, Taylor & Francis Books. 287-324.

Degrees

1978	B.S., University of California, Los Angeles, Biochemistry

1984	Ph.D., Stanford University, Pharmacology

Editorial Activities

Associate Editor

2008 - 2009

Journal of Alzheimer's Disease.

Editorial Board

2009 - present

International Journal of Alzheimer's Disease.

2009 - present

Journal of Biological Chemistry.

Honors

2004	Elizabeth Gross award for excellence in biophysics teaching, mentoring and research.

2009	Alzheimer's Disease Drug Discovery Foundation Award in recognition of innovative Research.

Inventions and Patents

Kuret, J., Khatami, S., Inventor, Patent filing July 31, 2001 and Patent issued November 12, 2002 [filed and issued]. Methods for Inhibiting or reversing tau filament formation. 6479528.

Kuret, J., Chirita, C.N., Necula, M., Inventor, Patent filing February 18, 2004 and Patent issued February 06, 2007 [filed and issued]. Identifying inhibitors of intracellular protein fibrillization. 7,172,875.

Memberships

2005 - present

Member. Society for Neuroscience

Peer Reviewed Journal Articles

Brown, J; Heininger, D; Kuret, J; Mullen, Y. 1981. Islet cells grow after transplantation of fetal pancreas and control of diabetes. Diabetes. Vol. 30, no. 1: 9-13.

BROWN, J; HEININGER, D; KURET, J; MULLEN Y. 1982. Normal Response To Pregnancy In Rats Cured Of Streptozotocin Diabetes By Transplantation Of 1 Fetal Pancreas. Diabetologia. Vol. 22, no. 4: 273-275.

Kuret, J; Schulman, H. 1984. Purification and characterization of a Ca2+/calmodulin-dependent protein kinase from rat brain. Biochemistry. Vol. 23, no. 23: 5495-504.

KURET, J; SCHULMAN H. 1985. Mechanism Of Autophosphorylation Of The Multifunctional Calcium-calmodulin-dependent Protein Kinase. Journal of Biological Chemistry. Vol. 260, no. 10: 6427-6433.

KURET, J; WOODGETT J, R; COHEN P. 1985. Multisite Phosphorylation Of Glycogen Synthase Ec-2.4.1.11 From Rabbit Skeletal Muscle Identification Of The Sites Phosphorylated By Casein Kinase I Ec-2.7.1.37. European Journal of Biochemistry. Vol. 151, no. 1: 39-48.

Schulman, H; Kuret, J; Jefferson, A, B; Nose, P, S; Spitzer, K H. 1985. Ca2+/calmodulin-dependent microtubule-associated protein 2 kinase: broad substrate specificity and multifunctional potential in diverse tissues. Biochemistry. Vol. 24, no. 20: 5320-7.

HOLMES C F, B; KURET, J; CHISHOLM A A, K; COHEN P. 1986. Identification Of The Sites On Rabbit Skeletal Muscle Protein Phosphatase Inhibitor 2 Phosphorylated By Casein Kinase Ii. Biochimica et Biophysica Acta. Vol. 870, no. 3: 408-416.

KURET, J; BELL, H; COHEN P. 1986. Identification Of High Levels Of Protein Phosphatase-1 In Rat Liver Nuclei. Febs Letters. Vol. 203, no. 2: 197-202.

KURET, J; JOHNSON K, E; NICOLETTE, C; ZOLLER M J. 1988. Mutagenesis Of The Regulatory Subunit Of Yeast Cyclic Amp-dependent Protein Kinase Isolation Of Site-directed Mutants With Altered Binding Affinity For Catalytic Subunit. Journal of Biological Chemistry. Vol. 263, no. 19: 9149-9154.

LEVIN L, R; KURET, J; JOHNSON K, E; POWERS, S; CAMERON, S; MICHAELI, T; WIGLER, M; ZOLLER M J. 1988. A Mutation In The Catalytic Subunit Of Cyclic Amp-dependent Protein Kinase That Disrupts Regulation. Science (Washington D C). Vol. 240, no. 4848: 68-70.

ZOLLER M, J; KURET, J; CAMERON, S; LEVIN, L; JOHNSON K E. 1988. Purification And Characterization Of C-1 The Catalytic Subunit Of Saccharomyces-cerevisiae Cyclic Amp-dependent Protein Kinase Encoded By Tpk1. Journal of Biological Chemistry. Vol. 263, no. 19: 9142-9148.

KURET, J; PFLUGRATH J W. 1991. Crystallization And Preliminary X-ray Analysis Of The Camp-dependent Protein Kinase Catalytic Subunit From Saccharomyces-cerevisiae. Biochemistry. Vol. 30, no. 43: 10595-10600.

CARMEL, G; KURET J. 1992. A Solid-phase Screen For Protein Kinase Substrate Selectivity. Analytical Biochemistry. Vol. 203, no. 2: 274-280.

WANG P-C; VANCURA, A; MITCHESON T G, M; KURET J. 1992. Two Genes In Saccharomyces-cerevisiae Encode A Membrane-bound Form Of Casein Kinase-1. Molecular Biology of the Cell. Vol. 3, no. 3: 275-286.

Vancura, Ales; O'Connor, Adrienne; Patterson, Scott D.; Mirza, Urooj; Chait, Brian T.; Kuret, Jeff. 1993. Isolation and properties of Yck2, a Saccharomyces cerevisiae homolog of casein kinase-1. Archives of Biochemistry and Biophysics. Vol. 305, no. 1: 47-53.

Carmel, Gilles; Leichus, Betty; Cheng, Xiaodong; Patterson, Scott D.; Mirza, Urooj; Chait, Brian T.; Kuret, Jeff. 1994. Expression, purification, crystallization, and preliminary X-ray analysis of casein kinase-1 from Schizosaccharomyces pombe. Journal of Biological Chemistry. Vol. 269, no. 10: 7304-7309.

Hoekstra, Merl F.; Dhillon, Namrita; Carmel, Gilles; Demaggio, Anthony J.; Lindberg, Richard A.; Hunter, Tony; Kuret, Jeff. 1994. Budding and fission yeast casein kinase I isoforms have dual-specificity protein kinase activity. Molecular Biology of the Cell. Vol. 5, no. 8: 877-886.

Kearney, Paul H.; Ebert, Michael; Kuret, Jeff. 1994. Molecular cloning and sequence analysis of two novel fission yeast casein kinase-1 isoforms. Biochemical and Biophysical Research Communications. Vol. 203, no. 1: 231-236.

Vancura, Ales; Sessler, Anna; Leichus, Betty; Kuret, Jeff. 1994. A prenylation motif is required for plasma membrane localization and biochemical function of casein kinase I in budding yeast. Journal of Biological Chemistry. Vol. 269, no. 30: 19271-19278.

Wang, Pi-Chao; Vancura, Ales; Desai, Arshad; Carmel, Gilles; Kuret, Jeff. 1994. Cytoplasmic forms of fission yeast casein kinase-01 associate primarily with the particulate fraction of the cell. Journal of Biological Chemistry. Vol. 269, no. 16: 12014-12023.

Xu, Rui-Ming; Carmel, Gilles; Sweet, Robert M.; Kuret, Jeff; Cheng, Xiaodong. 1995. Crystal structure of casein kinase-1, a phosphate-directed protein kinase. EMBO (European Molecular Biology Organization) Journal. Vol. 14, no. 5: 1015-1023.

Carmel, Gilles; Mager, Edward M.; Binder, Lester I.; Kuret, Jeff. 1996. The structural basis of monoclonal antibody Alz50's selectivity for Alzheimer's disease pathology. Journal of Biological Chemistry. Vol. 271, no. 51: 32789-32795.

Wang, Xiangmin; Hoekstra, Merl F.; Demaggio, Anthony J.; Dhillon, Namrita; Vancura, Ales; Kuret, Jeff; Johnston, Gerald C.; Singer, Richard A. 1996. Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase. Molecular and Cellular Biology. Vol. 16, no. 10: 5375-5385.

Xu, Rui-Ming; Carmel, Gilles; Kuret, Jeff; Cheng, Xiaodong. 1996. Structural basis for selectivity of the isoquinoline sulfonamide family of protein kinase inhibitors. Proceedings of the National Academy of Sciences of the United States of America. Vol. 93, no. 13: 6308-6313.

Kuret, Jeff; Johnson, Ginger S.; Cha, Donald; Christenson, Erik R.; Demaggio, Anthony J.; Hoekstra, Merl F. 1997. Casein kinase 1 is tightly associated with paired-helical filaments isolated from Alzheimer's disease brain. Journal of Neurochemistry. Vol. 69, no. 6: 2506-2515.

Murthy, S. N. Prasanna; Wilson, James H.; Lukas, Thomas J.; Kuret, Jeff; Lorand, Laszlo. 1998. Cross-linking sites of the human tau protein, probed by reactions with human transglutaminase. Journal of Neurochemistry. Vol. 71, no. 6: 2607-2614.

Bian, K; Davis, K; Kuret, J; Binder, L; Murad, F. 1999. Nitrotyrosine formation with endotoxin-induced kidney injury detected by immunohistochemistry. The American journal of physiology. Vol. 277, no. 1 Pt 2: F33-40.

Ghoshal, Nupur; Smiley, John F.; DeMaggio, Anthony J.; Hoekstra, Merl F.; Cochran, Elizabeth J.; Binder, Lester I.; Kuret, Jeff. 1999. A new molecular link between the fibrillar and granulovacuolar lesions of Alzheimer's disease. American Journal of Pathology. Vol. 155, no. 4: 1163-1172.

King, Michelle E.; Ahuja, Vibha; Binder, Lester I.; Kuret, Jeff. 1999. Ligand-dependent tau filament formation: Implications for Alzheimer's disease progression. Biochemistry. Vol. 38, no. 45: 14851-14859.

Sontag, Estelle; Nunbhakdi-Craig, Viyada; Lee, Gloria; Brandt, Roland; Kamibayashi, Craig; Kuret, Jeffrey; White, Charles L., III; Mumby, Marc C.; Bloom, George S. 1999. Molecular interactions among protein phosphatase 2a, tau, and microtubules: Implications for the regulation of tau phosphorylation and the development of tauopathies. Journal of Biological Chemistry. Vol. 274, no. 36: 25490-25498.

Tucholski, Janusz; Kuret, Jeff; Johnson, Gail V. W. 1999. Tau is modified by tissue transglutaminase in situ: Possible functional and metabolic effects of polyamination. Journal of Neurochemistry. Vol. 73, no. 5: 1871-1880.

Vancurova, Ivana; Choi, Jae H.; Lin, Hongyu; Kuret, Jeff; Vancura, Ales. 1999. Regulation of phosphatidylinositol 4-phosphate 5-kinase from Schizosaccharomyces pombe by casein kinase I. Journal of Biological Chemistry. Vol. 274, no. 2: 1147-1155.

Abraha, Aida; Ghoshal, Nupur; Gamblin, T. Chris; Cryns, Vincent; Berry, Robert W.; Kuret, Jeff; Binder, Lester I. 2000. C-terminal inhibition of tau assembly in vitro and in Alzheimer's disease. Journal of Cell Science. Vol. 113, no. 21: 3737-3745.

Gamblin, T. Chris; King, Michelle E.; Dawson, Hana; Vitek, Michael P.; Kuret, Jeff; Berry, Robert W.; Binder, Lester I. 2000. In vitro polymerization of tau protein monitored by laser light scattering: Method and application to the study of Ftdp-17 mutants. Biochemistry. Vol. 39, no. 20: 6136-6144.

Gamblin, T. Chris; King, Michelle E.; Kuret, Jeff; Berry, Robert W.; Binder, Lester I. 2000. Oxidative regulation of fatty acid-induced tau polymerization. Biochemistry. Vol. 39, no. 46: 14203-14210.

King, Michelle E.; Gamblin, T. Chris; Kuret, Jeff; Binder, Lester I. 2000. Differential assembly of human tau isoforms in the presence of arachidonic acid. Journal of Neurochemistry. Vol. 74, no. 4: 1749-1757.

Mashhoon, N; DeMaggio, A, J; Tereshko, V; Bergmeier, S, C; Egli, M; Hoekstra, M, F; Kuret, J. 2000. Crystal structure of a conformation-selective casein kinase-1 inhibitor. The Journal of biological chemistry. Vol. 275, no. 26: 20052-60.

Schwab, C; DeMaggio, A, J; Ghoshal, N; Binder, L, I; Kuret, J; McGeer, P L. 2000. Casein kinase 1 delta is associated with pathological accumulation of tau in several neurodegenerative diseases. Neurobiology of aging. Vol. 21, no. 4: 503-10.

Yasojima, Koji; Kuret, Jeff; DeMaggio, Anthony J.; McGeer, Edith; McGeer, Patrick L. 2000. Casein kinase 1 delta mrna is upregulated in Alzheimer disease brain. Brain Research. Vol. 865, no. 1: 116-120.

Ghoshal, Nupur; Garcia-Sierra, Francisco; Fu, Yifan; Beckett, Laurel A.; Mufson, Elliott J.; Kuret, Jeff; Berry, Robert W.; Binder, Lester I. 2001. Tau-66: Evidence for a novel tau conformation in Alzheimer's disease. Journal of Neurochemistry. Vol. 77, no. 5: 1372-1385.

Mashhoon, Neda; Carmel, Gilles; Pflugrath, James W.; Kuret, Jeff. 2001. Structure of the unliganded camp-dependent protein kinase catalytic subunit from Saccharomyces cerevisiae. Archives of Biochemistry and Biophysics. Vol. 387, no. 1: 11-19.

Yu, Jiang-Zhou; Kuret, Jeff; Rasenick, Mark M. 2002. Transient expression of fluorescent tau proteins promotes process formation in Pc12 cells: Contributions of the tau C-terminus to this process. Journal of Neuroscience Research. Vol. 67, no. 5: 625-633.

Chirita, Carmen N.; Necula, Mihaela; Kuret, Jeff. 2003. Anionic micelles and vesicles induce tau fibrillization in vitro. Journal of Biological Chemistry. Vol. 278, no. 28: 25644-25650.

Necula, Mihaela; Chirita, Carmen N.; Kuret, Jeff. 2003. Rapid anionic micelle-mediated alpha-synuclein fibrillization in vitro. Journal of Biological Chemistry. Vol. 278, no. 47: 46674-46680.

Chirita, Carmen, N; Kuret, Jeff. 2004. Evidence for an intermediate in tau filament formation. Biochemistry. Vol. 43, no. 6: 1704-14.

Chirita, Carmen; Necula, Mihaela; Kuret, Jeff. 2004. Ligand-dependent inhibition and reversal of tau filament formation. Biochemistry. Vol. 43, no. 10: 2879-2887.

Li, Guibin; Yin, Haishan; Kuret, Jeff. 2004. Casein kinase 1 delta phosphorylates tau and disrupts its binding to microtubules. Journal of Biological Chemistry. Vol. 279, no. 16: 15938-15945.

Necula, Mihaela; Kuret, Jeff. 2004. A static laser light scattering assay for surfactant-induced tau fibrillization. Analytical Biochemistry. Vol. 333, no. 2: 205-215.

Necula, Mihaela; Kuret, Jeff. 2004. Electron microscopy as a quantitative method for investigating tau fibrillization. Analytical Biochemistry. Vol. 329, no. 2: 238-246.

Necula, Mihaela; Kuret, Jeff. 2004. Pseudophosphorylation and glycation of tau protein enhance but do not trigger fibrillization in vitro. Journal of Biological Chemistry. Vol. 279, no. 48: 49694-49703.

Chirita, Carmen N.; Congdon, Erin E.; Yin, Haishan; Kuret, Jeff. 2005. Triggers of full-length tau aggregation: A role for partially folded interrnediates. Biochemistry. Vol. 44, no. 15: 5862-5872.

Kuret, Jeff; Chirita, Carmen N.; Congdon, Erin E.; Kannanayakal, Theresa; Li, Guibin; Necula, Mihaela; Yin, Haishan; Zhong, Qi. 2005. Pathways of tau fibrillization. Biochimica et Biophysica Acta. Vol. 1739, no. 2-3: 167-178.

Kuret, Jeff; Congdon, Erin, E; Li, Guibin; Yin, Haishan; Yu, Xian; Zhong, Qi. 2005. Evaluating triggers and enhancers of tau fibrillization. Microscopy research and technique. Vol. 67, no. 3-4: 141-55.

Necula, Mihaela; Chirita, Carmen N.; Kuret, Jeff. 2005. Cyanine dye N744 inhibits tau fibrillization by blocking filament extension: Implications for the treatment of tauopathic neurodegenerative diseases. Biochemistry. Vol. 44, no. 30: 10227-10237.

Necula, Mihaela; Kuret, Jeff. 2005. Site-specific pseudophosphorylation modulates the rate of tau filament dissociation. FEBS Letters. Vol. 579, no. 6: 1453-1457.

Kannanayakal, Theresa J.; Tao, Haiyang; Vandre, Dale D.; Kuret, Jeff. 2006. Casein kinase-1 isoforms differentially associate with neurofibrillary and granulovacuolar degeneration lesions. Acta Neuropathologica. Vol. 111, no. 5: 413-421.

Yin, Haishan; Kuret, Jeff. 2006. C-terminal truncation modulates both nucleation and extension phases of tau fibrillization. FEBS Letters. Vol. 580, no. 1: 211-215.

Yin, Haishan; Laguna, Kelly A.; Li, Guibin; Kuret, Jeff. 2006. Dysbindin structural homologue Ck1bp is an isoform-selective binding partner of human casein kinase-1. Biochemistry. Vol. 45, no. 16: 5297-5308.

Honson, Nicolette S.; Jensen, Jordan R.; Darb, Michael V.; Kuret, Jeff. 2007. Potent inhibition of tau fibrillization with a multivalent ligand. Biochemical and Biophysical Research Communications. Vol. 363, no. 1.

Bandyopadhyay, Bhaswati; Li, Guibin; Yin, Haishan; Kuret, Jeff. 2007. Tau aggregation and toxicity in a cell culture model of tauopathy. The Journal of biological chemistry. Vol. 282, no. 22. (June 1): 16454-64. (IF: 5.808)

Kuret, J. 2007. New Treatments for Alzheimer's Disease: How Close are We?. Aging Health. Vol. 4. (July): 445-448.

Congdon, Erin, E; Necula, Mihaela; Blackstone, Robert, D; Kuret, Jeff. 2007. Potency of a tau fibrillization inhibitor is influenced by its aggregation state. Archives of biochemistry and biophysics. Vol. 465, no. 1. (September 1): 127-35. (IF: 2.969)

Honson, N.S., Johnson, R.L., Huang, W., Inglese, J., Austin, C.P., and Kuret, J. 2007. Differentiating Alzheimer Disease-Associated Aggregates with Small Molecules. Neurobiology of Disease. Vol. 28. (December 7): 251-260. (IF: 4.128)

Chang E, Kim S, Yin H, Nagaraja HN, Kuret J. 2008. Pathogenic missense MAPT mutations differentially modulate tau aggregation propensity at nucleation and extension steps. J. Neurochem.. Vol. 107(4):1113-23.

Chang E, Kuret J. 2008. Detection and Quantification of Tau Aggregation Using a Membrane Filter Assay. Anal. Biochem.. Vol. 373(2):330-6.

Congdon EE, Kim S, Bonchak J, Songrug T, Matzavinos A, Kuret J. 2008. Nucleation-dependent tau filament formation - The importance of dimerization and an estimation of elementary rate constants. Journal of Biological Chemistry. Vol. 283(20):13806-16.

Honson NS, Kuret J. 2008. Tau aggregation and toxicity in tauopathic neurodegenerative diseases. J. Alzheimer's Dis.. Vol. 14(4):417-22.

Kannanayakal TJ, Mendell JR, Kuret J. 2008. Casein kinase 1 alpha associates with the tau-bearing lesions of inclusion body myositis. Neuroscience Letters. Vol. 431(2):141-5.

Sarker M, Kuret J, Lee G. 2008. Two motifs within the tau microtubule-binding domain mediate its association with the hsc70 molecular chaperone. J. Neurosci. Res.. Vol. 86(12):2763-73.

Trojanowski JQ, Duff K, Fillit H, Koroshetz W, Kuret J, Murphy D, Refolo L. 2008. New Directions for Frontotemporal Dementia Drug Discovery. Alzheimer's & Dementia. Vol. 4(2):89-93.

Chang, E., Congdon, E.E., Honson, N.S., Duff, K.E., Kuret J. 2009. Structure Activity Relationship of Cyanine Tau Aggregation Inhibitors. J. Med. Chem.. Vol. 52, no. 11: 3539-3547.

Chang, E., Honson, N.S., Bandyopadhyay, B., Funk, K.E., Jensen, J.R., Kim, S., Naphade, S., & Kuret, J. 2009. Modulation and detection of tau aggregation with small-molecule ligands. Curr. Alzheimer Res.. Vol. 6, no. 5: in press.

Congdon, E.E., Figueroa, Y.H., Wang, L., Tonuva, G., Chang, E., Kuret, J., Conrad, C., & Duff, K.E. 2009. Inhibition of tau polymerization with a cyanine dye in two distinct model systems. J. Biol. Chem.. Vol. 284: in press.

Honson, N.S., Jensen, J.R., Abraha, A, Hall, G.F., and Kuret, J. 2009. Small-molecule mediated neuroprotectionin an in situ model of tauopathy. Neurtox. Res.. Vol. 15, no. 3. (April): 274-283.

Positions

1984 - 1986

Postdoctoral Researcher, Medical Sciences Institute, Dundee University. United Kingdom.

1988 - 1990

Staff Investigator, Cold Spring Harbor Laboratory. NY.

1990 - 1994

Senior Staff Investigator, Cold Spring Harbor Laboratory. NY.

1995 - 1998

Investigator, Northwestern University Alzheimer's Disease Center. IL.

1998 - 2002

Associate Professor, The Ohio State University, Department of Molecular and Cellular Biochemistry. Columbus, OH, United States.

2002 - present

Professor, The Ohio State University, Medicine, Molecular & Cellular Biochemistry. Columbus, OH, United States.

Professional Activities

Consultation

1998 - present

Member, Initial Review Board of the Medical and Scientific Advisory Council. Chicago, IL.

2006 - present

Member, NIH Center for Scientific Review, Synapse, Cytoskeleton and trafficking Study Section (SYN). Washington, DC.

2007 - present

Member, NIH Center for Scientific Review, Special Emphasis Panel: Drug Discovery (MNPS-C). Washington, DC.