OSU:pro

Current OSU Appointment

Professor, Microbiology Administration

Degrees

1985	Ph.D., University of Wisconsin

Honors

Chair, Gordon Research Conference: "Molecular Basis of Microbial C1 Metabolism".

2005	Distinguished Scholar Award.

2007	American Academy of Microbiology Fellow.

Peer Reviewed Journal Articles

Krzycki, J, A; Wolkin, R, H; Zeikus, J G. 1982. Comparison of unitrophic and mixotrophic substrate metabolism by acetate-adapted strain of Methanosarcina barkeri. Journal of bacteriology. Vol. 149, no. 1: 247-54.

Krzycki, J, A; Zeikus, J G. 1984. Characterization and purification of carbon monoxide dehydrogenase from Methanosarcina barkeri. Journal of bacteriology. Vol. 158, no. 1: 231-7.

Krzycki, J, A; Lehman, L, J; Zeikus, J G. 1985. Acetate catabolism by Methanosarcina barkeri: evidence for involvement of carbon monoxide dehydrogenase, methyl coenzyme M, and methylreductase. Journal of bacteriology. Vol. 163, no. 3: 1000-6.

Zeikus, J, G; Kerby, R; Krzycki, J A. 1985. Single-carbon chemistry of acetogenic and methanogenic bacteria. Science (New York, N.Y.). Vol. 227, no. 4691: 1167-73.

Krzycki, J, A; Mortenson, L, E; Prince, R C. 1989. Paramagnetic centers of carbon monoxide dehydrogenase from aceticlastic Methanosarcina barkeri. The Journal of biological chemistry. Vol. 264, no. 13: 7217-21.

Lunnen, K, D; Morgan, R, D; Timan, C, J; Krzycki, J, A; Reeve, J, N; Wilson, G G. 1989. Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei. Gene. Vol. 77, no. 1: 11-9.

Reeve, J, N; Beckler, G, S; Cram, D, S; Hamilton, P, T; Brown, J, W; Krzycki, J, A; Kolodziej, A, F; Alex, L; Orme-Johnson, W, H; Walsh, C T. 1989. A hydrogenase-linked gene in Methanobacterium thermoautotrophicum strain delta H encodes a polyferredoxin. Proceedings of the National Academy of Sciences of the United States of America. Vol. 86, no. 9: 3031-5.

Sandman, K; Krzycki, J, A; Dobrinski, B; Lurz, R; Reeve, J N. 1990. HMf, a DNA-binding protein isolated from the hyperthermophilic archaeon Methanothermus fervidus, is most closely related to histones. Proceedings of the National Academy of Sciences of the United States of America. Vol. 87, no. 15: 5788-91.

Cao, X, J; Krzycki, J A. 1991. Acetate-dependent methylation of two corrinoid proteins in extracts of Methanosarcina barkeri. Journal of bacteriology. Vol. 173, no. 17: 5439-48.

Kremer, J, D; Cao, ; XKrzycki, J. 1993. Isolation of two novel corrinoid proteins from acetate-grown Methanosarcina barkeri. Journal of bacteriology. Vol. 175, no. 15: 4824-33.

Burke, S, A; Krzycki, J A. 1995. Involvement of the "A" isozyme of methyltransferase II and the 29-kilodalton corrinoid protein in methanogenesis from monomethylamine. Journal of bacteriology. Vol. 177, no. 15: 4410-6.

Ferguson, D J Jr; Krzycki, J, A; Grahame, D A. 1996. Specific roles of methylcobamide:coenzyme M methyltransferase isozymes in metabolism of methanol and methylamines in Methanosarcina barkeri. The Journal of biological chemistry. Vol. 271, no. 9: 5189-94.

Paul, L; Krzycki, J A. 1996. Sequence and transcript analysis of a novel Methanosarcina barkeri methyltransferase II homolog and its associated corrinoid protein homologous to methionine synthase. Journal of bacteriology. Vol. 178, no. 22: 6599-607.

Tallant, T, C; Krzycki, J A. 1996. Coenzyme M methylase activity of the 480-kilodalton corrinoid protein from Methanosarcina barkeri. Journal of bacteriology. Vol. 178, no. 5: 1295-301.

Burke, S, A; Krzycki, J A. 1997. Reconstitution of Monomethylamine:Coenzyme M methyl transfer with a corrinoid protein and two methyltransferases purified from Methanosarcina barkeri. The Journal of biological chemistry. Vol. 272, no. 26: 16570-7.

Ferguson, D J Jr; Krzycki, J A. 1997. Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri. Journal of bacteriology. Vol. 179, no. 3: 846-52.

Tallant, T, C; Krzycki, J A. 1997. Methylthiol:coenzyme M methyltransferase from Methanosarcina barkeri, an enzyme of methanogenesis from dimethylsulfide and methylmercaptopropionate. Journal of bacteriology. Vol. 179, no. 22: 6902-11.

Burke, S, A; Lo, S, L; Krzycki, J A. 1998. Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine. Journal of bacteriology. Vol. 180, no. 13: 3432-40.

Ferguson, D J Jr; Gorlatova, N; Grahame, D, A; Krzycki, J A. 2000. Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri. The Journal of biological chemistry. Vol. 275, no. 37: 29053-60.

Paul, L; Ferguson, D J Jr; Krzycki, J A. 2000. The trimethylamine methyltransferase gene and multiple dimethylamine methyltransferase genes of Methanosarcina barkeri contain in-frame and read-through amber codons. Journal of bacteriology. Vol. 182, no. 9: 2520-9.

James, C, M; Ferguson, T, K; Leykam, J, F; Krzycki, J A. 2001. The amber codon in the gene encoding the monomethylamine methyltransferase isolated from Methanosarcina barkeri is translated as a sense codon. The Journal of biological chemistry. Vol. 276, no. 36: 34252-8.

Tallant, T, C; Paul, L; Krzycki, J A. 2001. The MtsA subunit of the methylthiol:coenzyme M methyltransferase of Methanosarcina barkeri catalyses both half-reactions of corrinoid-dependent dimethylsulfide: coenzyme M methyl transfer. The Journal of biological chemistry. Vol. 276, no. 6: 4485-93.

Galagan, James, E; Nusbaum, Chad; Roy, Alice; Endrizzi, Matthew, G; Macdonald, Pendexter; FitzHugh, Will; Calvo, Sarah; Engels, Reinhard; Smirnov, Serge; Atnoor, Deven; Brown, Adam; Allen, Nicole; Naylor, Jerome; Stange-Thomann, Nicole; DeArellano, Kurt; Johnson, Robin; Linton, Lauren; McEwan, Paul; McKernan, Kevin; Talamas, Jessica; et al. 2002. The genome of M. acetivorans reveals extensive metabolic and physiological diversity. Genome research. Vol. 12, no. 4: 532-42.

Hao, Bing; Gong, Weimin; Ferguson, Tsuneo, K; James, Carey, M; Krzycki, Joseph, A; Chan, Michael K. 2002. A new UAG-encoded residue in the structure of a methanogen methyltransferase. Science (New York, N.Y.). Vol. 296, no. 5572: 1462-6.

Srinivasan, Gayathri; James, Carey, M; Krzycki, Joseph A. 2002. Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA. Science (New York, N.Y.). Vol. 296, no. 5572: 1459-62.

Blight, Sherry, K; Larue, Ross, C; Mahapatra, Anirban; Longstaff, David, G; Chang, Edward; Zhao, Gang; Kang, Patrick, T; Green-Church, Kari, B; Chan, Michael, K; Krzycki, Joseph A. 2004. Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo. Nature. Vol. 431, no. 7006: 333-5.

Hao, Bing; Zhao, Gang; Kang, Patrick, T; Soares, Jitesh, A; Ferguson, Tsuneo, K; Gallucci, Judith; Krzycki, Joseph, A; Chan, Michael K. 2004. Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid. Chemistry & biology. Vol. 11, no. 9: 1317-24.

Krzycki, Joseph A. 2004. Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases. Current opinion in chemical biology. Vol. 8, no. 5: 484-91.

Krzycki, Joseph A. 2005. The direct genetic encoding of pyrrolysine. Current opinion in microbiology. Vol. 8, no. 6: 706-12.

Soares, Jitesh, A; Zhang, Liwen; Pitsch, Rhonda, L; Kleinholz, Nanette, M; Jones, R Benjamin; Wolff, Jeremy, J; Amster, Jon; Green-Church, Kari, B; Krzycki, Joseph A. 2005. The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases. The Journal of biological chemistry. Vol. 280, no. 44: 36962-9.

Mahapatra, Anirban; Patel, Asmita; Soares, Jitesh, A; Larue, Ross, C; Zhang, Jun Kai; Metcalf, William, W; Krzycki, Joseph A. 2006. Characterization of a Methanosarcina acetivorans mutant unable to translate UAG as pyrrolysine. Molecular microbiology. Vol. 59, no. 1: 56-66.

Longstaff, David, G; Larue, Ross, C; Faust, Joseph, E; Mahapatra, Anirban; Zhang, Liwen; Green-Church, Kari, B; Krzycki, Joseph A. 2007. A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine. Proceedings of the National Academy of Sciences of the United States of America. Vol. 104, no. 3: 1021-6.